Fig. S7

Formation of secondary islets and apoptosis levels in pancreas are unaffected in nol9^{sa1022/sa1022} mutants.

(A) Representative confocal images of the pancreas of 120 hpf Tg(ptf1a:EGFP;ins:mCherry) larvae showing the presence of ins-expressing secondary islets (arrow) in nol9^{sa1022/sa1022} mutants and wt siblings. (B) The percentage of Tg(ptf1a:EGFP;ins:mCherry) larvae whose pancreas contained secondary islets, depending on their genotype and previous treatment with either DAPT inhibitor or DMSO (vehicle control) at 120 hpf. The total number of larvae in each group is indicated. (C) Representative confocal images of Tg(ptf1a:EGFP) larvae subjected to TUNEL assay at 96 hpf and co-stained with DAPI. No TMR-labelled apoptotic cells were observed in the ptf1a-expressing exocrine pancreas of nol9^{sa1022/sa1022} mutants (n = 8) or their wt siblings (n = 9). However, the tails of nol9^{sa1022/sa1022} mutants and wt siblings contained similar numbers of apoptotic cells (arrow). (D) The mean number of apoptotic cells in the tails of nol9^{sa1022/sa1022} mutants (n = 8) and wt siblings (n = 9). Data are represented as the mean +/- SEM; Student’s t-test, ns–not significant.