FIGURE

Fig. 2

ID

ZDB-FIG-170208-10

Publication

Bielczyk-Maczyńska et al., 2015 - The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish

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Fig. 2

Loss-of-function nol9 mutation leads to a defect in ITS2 pre-rRNA processing.

(A) Expression pattern of nol9 by WISH at sphere stage (4 hpf), 12 hpf, 48 hpf, 72 hpf, 96 hpf and 120 hpf. Black arrows indicate branchial arches, white arrow indicates pancreas and arrowhead indicates nol9-expressing cells in the CHT. (B) Representative Northern blot analysis of RNA isolated from 5 dpf nol9^{sa102/sa10222} mutants and control (nol9^+/+ and nol9^+/sa1022) siblings using 5’ETS, ITS1 and ITS2 probes to detect rRNA processing intermediates. Corresponding rRNA intermediates (a, b, c, d) are indicated in B) and C). (C) Schematic representation of the rRNA intermediates detected in the Northern blot analysis. The sites of hybridization of the 5’ETS, ITS1 and ITS2 probes are indicated in red. (D) Methylene blue staining of the membrane was used to control for equal loading of RNA. wt–nol9^+/+/nol9^+/sa1022, mut–nol9^{sa1022/sa1022}.

Expression Data

Gene:	nol9
Fish:	WT
Anatomical Terms:	caudal hematopoietic tissue liver pancreas pharyngeal arch 3-7 whole organism
Stage Range:	Sphere to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nol9^{sa1022/sa1022}
Observed In:	cleavage in ITS2 between 5.8S rRNA and LSU-rRNA of tricistronic rRNA transcript (SSU-rRNA, 5.8S rRNA, LSU-rRNA) rRNA processing
Stage:	Day 5

Phenotype Detail

Acknowledgments

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