Fig. 5

nol9^{sa1022/sa1022} mutants show a decrease in proliferation of c-myb+ cells.

(A) Representative maximum projection confocal images showing HSPCs (GFP^dim, arrow) in the CHT of Tg(cd41:EGFP) larvae at 96 hpf. Larvae are oriented with anterior to the left and dorsal to the top. (B) The number of GFP^dim HSPCs observed in the CHT region of nol9^{sa1022/sa1022} mutants (n = 20) and their wt siblings (n = 51) at 96 hpf. Data are represented as the mean +/- SEM, Student’s t-test, **, p<0.01. (C) Representative confocal images of the CHT of Tg(cmyb:EGFP) embryos subjected to BrdU incorporation assay at 48 hpf. Arrowheads mark double positive BrdU⁺ c-myb⁺ cells. Bright field image of a 48 hpf embryo shows the imaged part of the CHT. (D) The total number of c-myb⁺ cells observed in the CHT of nol9^{sa1022/sa1022} embryos (n = 13) and their wt siblings (n = 14) subjected to the BrdU incorporation assay at 48 hpf. Data are represented as the mean +/- SEM. Student’s t-test. ns—not significant. (E) The percentage of BrdU⁺ cells within the c-myb⁺ population in the CHT of nol9^{sa1022/sa1022} embryos (n = 13) and their wt siblings (n = 14) subjected to the BrdU incorporation assay at 48 hpf. Data are represented as the mean +/- SEM, Student’s t-test, *, p<0.05.