FIGURE

Fig. 7

ID

ZDB-FIG-170208-15

Publication

Bielczyk-Maczyńska et al., 2015 - The Ribosome Biogenesis Protein Nol9 Is Essential for Definitive Hematopoiesis and Pancreas Morphogenesis in Zebrafish

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Fig. 7

The number of HSPCs is rescued in a tp53 mutant background.

(A) The CHT of 96 hpf larvae from a nol9^+/sa1022;tp53^+/zdf1 x nol9^+/sa1022;tp53^+/zdf1 cross stained by WISH against c-myb. Signal extracted from the corresponding WISH photograph is shown. Numbers represent larvae with the displayed phenotype out of the total number of larvae examined. (B) Quantification of c-myb in situ signal for 96 hpf larvae from a nol9^+/sa1022;tp53^+/zdf1 x nol9^+/sa1022;tp53^+/zdf1 cross, depending on their genotype. Data are represented as the mean number of pixels +/- SEM. nol9^+/+;tp53^+/+ n = 9; nol9^-/-;tp53^+/+ n = 11; nol9^-/-;tp53^+/zdf1 n = 19; nol9^-/-; tp53^zdf1/zdf1 n = 10. Two-tailed Student’s t-Test, *, p<0.05; **, p<0.01. (C) Representative pictures of the CHT of 96 hpf larvae from a nol9^+/sa1022;tp53^+/zdf1 x nol9^+/sa1022;tp53^+/zdf1 cross stained by WISH against hbae1. Signal extracted from the corresponding WISH photograph is shown. (D) Quantification of hbae1 WISH signal for larvae from a nol9^+/sa1022;tp53^+/zdf1 x nol9^+/sa1022;tp53^+/zdf1 cross, depending on their genotype. Data are represented as the mean number of pixels +/- SEM. nol9^+/+;tp53^+/+ n = 16; nol9^-/-;tp53^+/+ n = 14; nol9^-/-;tp53^+/- n = 23; nol9^-/-;tp53^-/- n = 16. Two-tailed Student’s t-Test, *, p<0.05; **, p<0.01. Within the figure, nol9^sa1022 allele has been denoted as nol9^- and tp53^zdf1 as tp53^-.

Expression Data

Genes:	hbae1.1 myb
Fish:	WT nol9^{sa1022/sa1022} nol9^{sa1022/sa1022}; tp53^zdf1/+ nol9^{sa1022/sa1022}; tp53^zdf1/zdf1
Anatomical Terms:	post-vent region erythroid progenitor cell post-vent region hematopoietic multipotent progenitor cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nol9^{sa1022/sa1022} nol9^{sa1022/sa1022}; tp53^zdf1/+ nol9^{sa1022/sa1022}; tp53^zdf1/zdf1
Observed In:	hematopoietic multipotent progenitor cell post-vent region erythroid progenitor cell post-vent region hematopoietic multipotent progenitor cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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