FIGURE

Fig. S4

ID

ZDB-FIG-170207-19

Publication

Bachmann-Gagescu et al., 2015 - The Ciliopathy Protein CC2D2A Associates with NINL and Functions in RAB8-MICAL3-Regulated Vesicle Trafficking

Other Figures

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Fig. S4

Rescue of the morphant phenotype supports its specificity.

(a-d) Co-injection of 2 ng ninl atgMO with 150 pg capped MO-resistant mRNA encoding human NINL isoform B reduced the incidence of body curvature defects from 71% in ninl atgMO injected larvae (n = 207) to 36% in ninl atgMO + NINL mRNA injected larvae (n = 203) (P<0.0001, two-tailed Fisher's exact). A subset of these larvae were sectioned and a perfect correlation was observed in rescue between body curvature defects and defects in photoreceptor outer segment formation (a'-c'). (e) Quantification of the rescue of retinal outer segment length showing that mean OS length was rescued from 1.6 +/- 0.26 μm in ninl morphants to 3.8 +/- 0.25 μm with co-injection of NINL mRNA (P<0.0001, unpaired Student's t-test).

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-ninl
Knockdown Reagent:	MO1-ninl
Observed In:	eye photoreceptor cell photoreceptor outer segment pericardium photoreceptor cell outer segment organization whole organism anterior-posterior axis
Stage:	Day 4

Phenotype Detail

Acknowledgments

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