Fig. 5

Ninl is required for correct Rab8A localization.

(a-a') Expression of a rhodopsin-promoter driven cherry-tagged Rab8a in wild-type photoreceptors is mostly concentrated in one or several puncta (arrows a-a') whereas it is diffuse in the majority of ninl morphant photoreceptors (b-b'). (c) Proportion of Rab8a-cherry expressing photoreceptors with punctate expression versus diffuse expression (bars represent 95% confidence interval; ** P<0.001, Fisher's exact test). (d-d') Endogenous Rab8a localization as seen by immunohistochemistry using an anti-Rab8a antibody (green) displays similar puncta (arrowheads) in wild-type photoreceptors, while the number of puncta is decreased in ninl morphant photoreceptors (e-e'). (f) Quantification of the number of Rab8a puncta displayed in the form of a scatter plot indicating that significantly fewer endogenous Rab8 puncta per μm² are present in ninl morphants compared to uninjected controls (*P = 0.01, unpaired Student's t-test; bars represent standard error of the mean). Scoring was performed blinded as to injection status for (c) and (f). Outer segments are counterstained with bodipy in (d-e). Nuclei are counterstained with DAPI. All images are cryosections of 4 dpf larvae. Scale bars are 4 μm in all panels.