ZFIN Image: Bachmann-Gagescu et al., 2015, Fig. S4

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Figure Caption

Fig. S4

Rescue of the morphant phenotype supports its specificity.

(a-d) Co-injection of 2 ng ninl atgMO with 150 pg capped MO-resistant mRNA encoding human NINL isoform B reduced the incidence of body curvature defects from 71% in ninl atgMO injected larvae (n = 207) to 36% in ninl atgMO + NINL mRNA injected larvae (n = 203) (P<0.0001, two-tailed Fisher's exact). A subset of these larvae were sectioned and a perfect correlation was observed in rescue between body curvature defects and defects in photoreceptor outer segment formation (a'-c'). (e) Quantification of the rescue of retinal outer segment length showing that mean OS length was rescued from 1.6 +/- 0.26 μm in ninl morphants to 3.8 +/- 0.25 μm with co-injection of NINL mRNA (P<0.0001, unpaired Student's t-test).

Figure Data

Phenotype details

Acknowledgments

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