Fig. S7
- ID
- ZDB-FIG-160818-48
- Publication
- Zhang et al., 2016 - Eph-ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer
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Effectiveness test of efnb2b and efnb2a morpholinos. (A) Illustration of efnb2b mRNA and reporter structures. The approximate positions of two morpholinos, MO1 and MO2, were indicated. (B) Effect of MO injection on the reporter expression. 75 pg of the reporter plasmid pefnb2b-5′UTR-GFP was injected alone or together with 10 ng efnb2b-MO1 or 7 ng efnb2b-MO2 into one-cell stage embryos. The injected embryos were observed at midgastrulation stages for GFP expression under a dissect fluorescence microscope. (C) Effect of efnb2b-MO2 on development. Embryos at the one-cell stage were injected with 7 ng efnb2b-MO2 or 7 ng cMO. The live embryos were observed at later stages as indicated. All embryos were orientated laterally. Embryos injected with efnb2b-MO developed slower than those injected with cMO. (D) Effect of efnb2a knockdown on Efnb2a protein level. Embryos were injected with a morpholino or mRNA species at the one-cell stage and harvested at the 60% epiboly stage for Western blotting using different antibodies. Note that anti-Efnb2 appears to only recognize Efnb2a protein. |