FIGURE

Fig. S3

ID

ZDB-FIG-160818-44

Publication

Zhang et al., 2016 - Eph-ephrin signaling maintains the boundary of dorsal forerunner cell cluster during morphogenesis of the zebrafish embryonic left-right organizer

Other Figures

All Figure Page

Back to All Figure Page

Fig. S3

Efficiency of mRNA and morpholino injection at midblastulation stages. (A,B) Injection of 250 pg ephb4b 5′utr-mCherry fusion mRNA, which contained 5′ untranslated sequence including the ephb4b-MO2 binding site, into one-cell Tg(sox17:GFP) transgenic embryos led to global expression of mCherry including DFCs. When Tg(sox17:GFP) embryos were first injected with 250 pg ephb4b 5′utr-mCherry mRNA at the one-cell stage and then injected with 10 ng ephb4b-MO2 at the 512-cell stage, mCherry in GFP-positive DFCs was drastically reduced while it was retained in surrounding cells, suggesting that ephb4b-MO2 was restricted to DFCs. (C) Injection of 400 pg ephb4b-mCherry mRNA, which contains full-length ephb4b coding sequence, into 256-cell to 512-cell Tg(sox17:GFP) embryos led to mCherry expression specifically in DFCs. All of injected embryos were observed by confocal microscopy focusing on the DFC region at the 75% epiboly stage. The ratio of embryos with representative pattern was indicated. Scale bar: 30 µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development