FIGURE

Fig. 2

ID

ZDB-FIG-160405-14

Publication

Jaffe et al., 2016 - c21orf59/kurly Controls Both Cilia Motility and Polarization

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Fig. 2

Kur Is a Cytoplasmic Factor Required for the Localization of ODAs to the Cilium

(A-D′) Transmission electron micrographs (TEMs) showing the ultrastructure of pronephric cilia in cross-section. The arrows in (A) and (A′) indicate ODAs that are missing in mutants (B-C′), but are restored in kur^tm304/tm304 mutants raised at permissive temperature (D and D′).

(E) IP of Myc-tagged Swt and subsequent Myc-Swt and FLAG-Kur western blot (WB) reveals physical association between Swt and Kur.

(F-G′) NIH 3T3 cells transfected with inducible Myc-tagged Kur were immunostained for the ciliary axoneme (acetylated alpha tubulin [ac-tub]; red), DNA (Hoechst; blue), and myc (Myc-Kur; green). The Kur is not localized to the cilium, but instead localizes to the cytoplasm.

(H-I′) Immunostaining of KV in control embryos or embryos injected with Myc-Kur. The cilia were marked by ac-tub (green) staining, DNA with Hoechst (blue), and Myc-Kur was localized with an anti-Myc antibody (red). The Myc-Kur, which rescues kur mutants, was present in cells of the KV, but appeared absent from cilia themselves.

Expression Data

Gene:	cfap298
Antibody:	Ab1-tuba
Fish:	WT
Anatomical Terms:	Kupffer's vesicle cytoplasm Kupffer's vesicle motile cilium
Stage:	5-9 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	cfap298^tj271/tj271 cfap298^tm304/tm304
Conditions:	cold exposure heat exposure
Observed In:	pronephric duct motile cilium pronephric duct outer dynein arm pronephric duct outer dynein arm assembly
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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