FIGURE

Fig. 4

ID

ZDB-FIG-160405-16

Publication

Jaffe et al., 2016 - c21orf59/kurly Controls Both Cilia Motility and Polarization

Other Figures

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Fig. 4

Kur Interacts with PCP Components

(A-C) Low concentration of inv or kur MO produce few defects, but, in combination, produce additive defects in body curvature and presence of kidney cysts. The white arrows show kidney cysts. The statistical comparison of different genotypes was performed using chi-square analysis with p < 0.05 being considered to report significant differences.

(D) IP of FLAG-tagged Dvl and subsequent FLAG-Dvl and HA-Kur western blot (WB) reveal physical association between Dvl and Kur.

(E) IP of Myc-tagged Dvl domains and subsequent Myc-Dvl and HA-Kur western blot (WB) reveal physical association between Kur and the DEP and PDZ domains, but not the DIX domain of Dvl.

(F) IP of FLAG-tagged Kur and subsequent FLAG-Kur and HA-Sea western blot (WB) reveal physical association between Sea and Kur.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-invs WT + MO3-cfap298 WT + MO1-invs + MO3-cfap298
Knockdown Reagents:	MO1-invs MO3-cfap298
Observed In:	post-vent region pronephros trunk
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

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