Fig. 3
Dysregulated Lysosomal Activity in Microglia of rragast77/st77 Mutants (A and B) Analysis of RNA sequencing data revealed that genes associated with lysosomal activity are significantly upregulated in rragast77/st77 mutants at 5 dpf. Using the DAVID Bioinformatics Resources, genes showing at least a 1.5-fold upregulation were classified according to (A) Kegg Pathway terms and (B) molecular functions (GOTERM_MF_FAT). (C and D) Visualization of Lysotracker Red and Tg(mpeg1:EGFP) in WT (C, n = 4) and rragast77/st77 mutants (D, n = 4) reveals that lysosomes are increased in microglia in the mutants. Larvae shown in (C) and (D) were genotyped by PCR after photography. |
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Stage: | Day 4 |
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Stage: | Day 4 |