Fig. S2
Related to Figure 2. rraga mRNA is maternally expressed and widely distributed in the embryo. (A) Time course of rraga mRNA expression analyzed by RTPCR of whole-animal lysates prepared at the indicated stages before (black) and after (red) the maternal-zygotic transition. RNA for rraga is detected before the zygotic transcription is active, in contrast to zygotically expressed control genes twist2 and plp1a. (B) Representative image of a 2 dpf embryo stained by in situ hybridization with antisense and sense probes for the rraga transcript. The antisense probe detected widespread expression, whereas the control sense probe showed background staining. |