ZFIN Image: Shen et al., 2016, Fig. 3

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Figure Caption

Fig. 3

Dysregulated Lysosomal Activity in Microglia of rraga^st77/st77 Mutants

(A and B) Analysis of RNA sequencing data revealed that genes associated with lysosomal activity are significantly upregulated in rraga^st77/st77 mutants at 5 dpf. Using the DAVID Bioinformatics Resources, genes showing at least a 1.5-fold upregulation were classified according to (A) Kegg Pathway terms and (B) molecular functions (GOTERM_MF_FAT).

(C and D) Visualization of Lysotracker Red and Tg(mpeg1:EGFP) in WT (C, n = 4) and rraga^st77/st77 mutants (D, n = 4) reveals that lysosomes are increased in microglia in the mutants. Larvae shown in (C) and (D) were genotyped by PCR after photography.

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Acknowledgments

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