FIGURE

Fig. 4

ID

ZDB-FIG-160308-5

Publication

Shen et al., 2016 - The Rag-Ragulator Complex Regulates Lysosome Function and Phagocytic Flux in Microglia

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Abnormal Vacuolar Organelles in Microglia and Uncleared Apoptotic Neurons in rraga^st77/st77 Mutants

(A–C) Transmission electron micrographs of the dorsal midbrain of (A) WT, (B and B′) rraga^st77/st77, and (C) irf8^st95/st95 larvae at 4 dpf. Boxed region in (B) is shown at higher magnification in (B′). Microglia in the rraga^st77/st77 mutants have a striking accumulation of vacuolar organelles that appear to contain undigested material (white arrows). Microglia were absent in the irf8 mutant. In both mutants, uncleared corpses of apoptotic neurons are present (white arrowheads). These phenotypes also were evident in mutants stained with acridine orange ( Figure S3). Toluidene blue-stained sections of the same larvae are shown in Figure S4. Larvae were genotyped by PCR from tail biopsies collected immediately prior to fixation.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	irf8^st95/st95 rraga^st77/st77
Observed In:	brain microglial cell microglial cell lysosome organization microglial cell phagolysosome optic tectum apoptotic cell clearance optic tectum microglial cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Cell Rep.