FIGURE

Fig. 6

ID

ZDB-FIG-160304-22

Publication

Ye et al., 2016 - An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration

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Fig. 6

Insulin knockdown enhances regeneration of β cells. (A) Schematic of β cell ablation and regeneration experiments. Control and insaMO-injected insa:CFP-NTR embryos were treated with MTZ from 52 to 80 hpf, recovered in fresh egg water for 1 day, and analyzed at ~100 hpf (1 day post ablation; dpa). (B–C′) Merged (B,C) and single channel (B′,C′) confocal projections of regenerated 100 hpf control (B,B′) and insaMO-injected (C,C′) islets immunostained for Gcg (red), CFP (β cells, green), and DNA (blue). Arrows indicate the expansion of strongly double positive Ins+ Gcg+ cells. (D–E′) Merged (D,E) and single channel (D′,E′) confocal projections of 100 hpf control (D,D′) and insaMO-injected (E,E′) islets immunostained for CFP (green) and DNA (blue). All islets expressed zygotically-injected H2B-RFP (red) to mark DBCs. Arrows indicate the expansion of double positive H2B-RFP+ Ins+ cells; this indicates conversion of non-β endocrine cells into β cells. (F) Quantification of insa+, Gcg+, and insa+ Gcg+ cells in 1 dpa control (n=17) and insaMO-injected (n=22) islets. (G) Quantification of H2B-RFP+ insa+ and H2B-RFP^-insa+ regenerating β cells in 1 dpa control (n=17) and insaMO-injected (n=13) islets. Student′s t-test was used in G to determine statistical significance.

Expression Data

Gene:	CFP
Fish:	s892Tg s892Tg + MO2-ins
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Knockdown Reagent:	MO2-ins
Anatomical Term:	regenerating tissue pancreatic B cell
Stage:	Day 4

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	s892Tg + MO2-ins
Condition:	chemical ablation: pancreatic B cell, chemical treatment by environment: metronidazole
Knockdown Reagent:	MO2-ins
Observed In:	pancreatic A cell regeneration pancreatic B cell regeneration regenerating tissue pancreatic B cell
Stage:	Day 4

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 409(2), Ye, L., Robertson, M.A., Mastracci, T.L., Anderson, R.M., An insulin signaling feedback loop regulates pancreas progenitor cell differentiation during islet development and regeneration, 354-69, Copyright (2016) with permission from Elsevier. Full text @ Dev. Biol.