FIGURE

Fig. 3

ID

ZDB-FIG-160229-13

Publication

Cao et al., 2016 - Single epicardial cell transcriptome sequencing identifies Caveolin-1 as an essential factor in zebrafish heart regeneration

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Fig. 3

Expression of the cav1:cav1-EGFP reporter. (A) Generation of the cav1:cav1-EGFP BAC reporter. The original 187kb BAC CH211-226K23 covers genes cav2, cav1 and met. The Cav1 translational stop codon (TAA) in exon 3 was replaced with an EGFP-SV40 poly(A) cassette with a 20 amino acid spacer, resulting in a C-terminal fusion protein. (B-E) Visualization of Cav1-EGFP reporter expression in embryos at 3dpf (B,C), 5dpf (D) and 6dpf (E), assessed in the flk1:DsRed2 (B,C,E) or tcf21:DsRed2 (D) background. (B-D) Stereofluorescence scope images; (E) confocal microscopy images. EGFP expression was detected in heart (arrowheads in B,D), blood vessels (B,C,E), notochord (C), branchial arch (D), skin (E), neuromast (E) and other tissues.

Expression Data

Gene:	EGFP
Fish:	pd1096Tg; pd27Tg pd1096Tg; pd37Tg
Anatomical Terms:	heart integument neuromast notochord pharyngeal arch 3-7 vasculature
Stage Range:	Protruding-mouth to Day 6

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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