Fig. S4

A second allele of cav1 disrupts heart regeneration. (A) The TALEN pairs were used to target to the sequences labeled in red, which is the common sequence of both isoforms. A mutant with a 23-nt deletion, cav1^pd1104, was identified, which leads to truncated proteins of both a and b isoforms lacking all the important domains for caveolae formation. (B) cav1^pd1104 heterozygous mutants (right) displayed severe defects in muscle regeneration (top) and fibrin/collagen retention (bottom). Section images of ventricles at 30 dpa are stained for MHC to denote cardiac muscle. Dashed line indicates plane of resection. The same sections in top panels are stained with acid fuchsin orange G to characterize non-muscle components in the injuries (bottom; blue for collagen, red for fibrin). (C) Quantification of regeneration scores between cav1 mutants and wildtype siblings were compared at 30 dpa. Data indicate the percent of total hearts represented by each score for each genotype. n = 9 for wild-types, and n = 16 for heterozygotes.