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Fig. 3
Expression of the cav1:cav1-EGFP reporter. (A) Generation of the cav1:cav1-EGFP BAC reporter. The original 187kb BAC CH211-226K23 covers genes cav2, cav1 and met. The Cav1 translational stop codon (TAA) in exon 3 was replaced with an EGFP-SV40 poly(A) cassette with a 20 amino acid spacer, resulting in a C-terminal fusion protein. (B-E) Visualization of Cav1-EGFP reporter expression in embryos at 3dpf (B,C), 5dpf (D) and 6dpf (E), assessed in the flk1:DsRed2 (B,C,E) or tcf21:DsRed2 (D) background. (B-D) Stereofluorescence scope images; (E) confocal microscopy images. EGFP expression was detected in heart (arrowheads in B,D), blood vessels (B,C,E), notochord (C), branchial arch (D), skin (E), neuromast (E) and other tissues.