FIGURE

Fig. 6

ID

ZDB-FIG-151123-8

Publication

Hamling et al., 2015 - Mapping the development of cerebellar purkinje cells in zebrafish

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Fig. 6

Vglut1 expression in parallel fibers moves anteriorly early in development and coincides with Purkinje cell development. (A, B) Expression at 2.9 dpf (70 hpf) of VGlut1 (A1, B1) and Pvalb7 (A2, B2), in both dorsal and sagittal views. Merge (A3, B3) shows that VGlut1 and Pvalb7 show little overlap at this early time point. Additionally, VGlut1 expression can be found in parallel fibers that project posteriorly (A1, B1; white arrowheads). (C, D) Expression at 3.3 dpf (80 hpf) of VGlut1 (C1, D1), Pvalb7 (C2, D2) and merge (C3, D3) shows that parallel fibers expand forward, beginning to overlap with Purkinje cells during this time period. There is substantial outgrowth of parallel fibers projecting posteriorly (C1, D1; white arrowheads). (E, F) Expression at 5dpf shows that by this time point VGlut1 (E1, F1) and Pvalb7 (E2, F2) are highly spatially coordinated (merge; E3, F3) and both layers have begun to curve anteriorly. Scale bar 50 µm. Images taken using 20× (1.0 NA) water objective.

Expression Data

Antibodies:	Ab1-pvalb7 Ab1-slc17a7
Fish:	AB/TL
Anatomical Terms:	hindbrain parallel fiber Purkinje cell
Stage Range:	Pec-fin to Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dev. Neurobiol.