Fig. 5

Irf4a-Deficient Lymphoid Progenitors Turn to Myeloid Cells

(A) Expression of coro1a in the siblings and irf4a mutants at 3 dpf. The black arrowhead indicates the thymus, and the red arrow indicates the coro1a-expressing cell in the CHT.

(B) Confocal images of coro1a:EGFP cells in the thymus (white circle) and CHT of the control and irf4a morphants at 3 dpf. Scale bar, 80 µm.

(C) Representative FACS analysis of whole-body coro1a:EGFP cells in the control and irf4a morphants at 3 dpf.

(D) Enumeration of whole-body coro1a:EGFP cells shown in (C). Data are representative of three independent experiments (n = 180). ns, not significant.

(E) Expression of mfap4 and lyz in the CHT of the control and irf4a morphants at 3 dpf. Red arrows indicate myeloid cells expressing mfap4 or lyz.

(F) Brief diagram of irf4a knockin construct and generation of transgenic line for single-cell tracing.

(G) Time-lapse imaging of the conversion of the irf4a-deficient cell with EGFP knockin to express lyz:DsRed in the CHT from 58 to 66 hpf. Scale bar, 10 µm.

(H) Confocal images of cmyb:EGFP/lyz:DsRed embryos showing cmyb:EGFP, lyz:DsRed, or yellow cells in the CHT of the control or irf4a morphants at 3 dpf. Scale bar, 40 µm.

(I) Enumeration of cmyb:EGFP, lyz:DsRed, or yellow cells shown in (H). p < 0.01; p < 0.05. All data are mean ± SD.

See also Figure S5 and Movie S2.