FIGURE
Fig. S2
Fig. S2
Validation of irf4a morpholinos and mutant. (A) The protein level of Irf4a in the control and irf4a MOatg at 2 dpf examined by western blot. (B) Expression of rag1 in the control and irf4a MOatg at 4 dpf examined by WISH. Red arrows indicate the thymus. (C) The schematic diagram for the evaluation of irf4a splice morpholino. The target of irf4a splice morpholino is denoted with red marker. (D) The amplification of 460 bp products by RT-PCR analysis on cDNA from 2 dpf embryos. Compared to the control, irf4a MOsp injected embryos showed reduced 460 bp PCR products. (E) Expression of rag1 in the control and embryos injected with splice morpholino or mismatch morpholino at 4 dpf. Red arrows indicate the thymus. (F) The schematic diagram of the preparation of irf4a mutant embryos. The embryos were obtained by an incross of genotyped heterozygous irf4a mutant and examined by WISH. (G) Expression of irf4a in the sibling and irf4a mutant at 2 dpf. Red arrows indicate irf4a-expressing cells in the CHT. |
Expression Data
Expression Detail
Antibody Labeling
Phenotype Data
Phenotype Detail
Acknowledgments
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Reprinted from Developmental Cell, 34(6), Wang, S., He, Q., Ma, D., Xue, Y., Liu, F., Irf4 Regulates the Choice between T Lymphoid-Primed Progenitor and Myeloid Lineage Fates during Embryogenesis, 621-31, Copyright (2015) with permission from Elsevier. Full text @ Dev. Cell