Fig. 2

slc26a2 mRNA expression in embryonic and adult zebrafish.

(A)RT-PCR analysis of slc26a2 expression in 0–96 h zebrafish yielded 405 bp products, GAPDH served as an internal control for cDNA quantification and gave a 275 bp product. mRNA expression of slc26a2 was detected in the first development period, and transcription remained high after 48 h. (B) Expression of slc26a2 in WT embryos at 12 and 26hpf, detected by in situ hybridization, at 12hpf. The slc26a2 transcript was expressed broadly, and at 26hpf, slc26a2 was expressed circumferentially around the ln, lateral line primordium; ot, otic vesicle; pf, pectoral fin, and mb, midbrain. This suggests a function for slc26a2 in the inner ear and neuromast development. Wild insets depict enlarged images of the otic vesicles and lateral neuromasts. (C) RT-PCR confirmed the effectiveness of S2-SP1, and specific products were amplified from cDNA. Wild-type zebrafish yielded 1256 bp products for slc26a2, and morphants had 516 bp products for S2-SP1 in addition to those observed in WT fish. Two bands appeared for morphants but only one band was seen for WT.