FIGURE

Fig. 6

ID

ZDB-FIG-151106-25

Publication

Liu et al., 2015 - Solute Carrier Family 26 Member a2 (slc26a2) Regulates Otic Development and Hair Cell Survival in Zebrafish

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Fig. 6

Knockdown of slc26a2 induces apoptotic signals.

Morphology of 120hpf WT AB line zebrafish embryos injected with morpholinos. One- or two-celled embryos were injected with 4 ng of S2-SP1 and Mcon MO (control). WT was injected with RNase-free water. Neuromast hair cells were measured with TUNEL and changes in apoptotic signals in the anterior and posterior lateral neuromasts occurred (scale bars = 10 µm). (B) Statistical analysis of the apoptotic hair cells in anterior and posterior lateral neuromasts in different types of embryos at 120hpf, including WT, S2-SP1 and Rescue, in order to exclude the possibility that the apoptosis hair cells of MO knockdown zebrafish were due to off-target MO toxicity, we co-injected S2-SP1 with p53-MO. ***P<0.001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-slc26a2 AB + MO1-slc26a2 + MO4-tp53
Knockdown Reagents:	MO1-slc26a2 MO4-tp53
Observed In:	anterior lateral line neuromast hair cell posterior lateral line neuromast hair cell
Stage:	Day 5

Phenotype Detail

Acknowledgments

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