Fig. S2

Evaluation of the knockdown efficiency of ddb1 morpholinos.

(A-B) RT-PCR to test the efficiency of ddb1 splice site targeted morpholino (ddb1_MO1). (A) Schematic diagram of ddb1_MO1 targeting the 5^th exon-intron junction. (B) Efficiency assay of ddb1_MO1 by RT-PCR using two pairs of ddb1-specific primers. The amount of RNA and cDNA used for RT-PCR was the same for the different samples and for the internal control β-actin. Negative control (con) contained no cDNA. (C-D) Evaluation of the knockdown efficiency of ddb1 translation start site morpholino (ddb1_MO2). (C) Schematic representation of ddb1-egfp fusion construct used for ddb1_MO2 efficiency assay. (D) The expression of EGFP in larvae injected with ddb1-egfp fusion construct together with or without ddb1_MO2. Anterior towards the left. Abbreviations used: E4/E5/E6, 4^th /5^th /6^th exon; I4/I5/I6, 4^th / 5^th / 6^th intron; ddb1_LF/R, the forward and reverse primers of longer ddb1-specific fragment; ddb1_SF/R, forward and reverse primers of shorter ddb1-specific fragment; 1 and 2, different samples; uninj, uninjected embryos.