FIGURE

Fig. 4

ID

ZDB-FIG-151008-18

Publication

Hu et al., 2015 - Loss of DDB1 Leads to Transcriptional p53 Pathway Activation in Proliferating Cells, Cell Cycle Deregulation, and Apoptosis in Zebrafish Embryos

Other Figures

All Figure Page

Back to All Figure Page

Fig. 4

Enhanced apoptosis in proliferation regions of the ddb1^m863 mutant CNS.

(A-F) The pcna expression pattern in ddb1^m863 mutants and wild type siblings at 30 hpf (A-B) and 72 hpf (C-F). (G-J) TUNEL assay for apoptosis in ddb1^m863 mutants (H-H′, J) and wild type siblings (G-G′, I) at 72 hpf. Embryos or larvae in lateral (A-B, E-H) and ventral views (C-D). Cross sections (20 µm; I-J) from larvae by TUNEL assay. Arrows represent affected pcna-expressing cells or enhanced apoptotic cells in the retina (D, J; red), pretectal region (F, J; blue), and cerebellum (F; green). Abbreviations used: ba, branchial arches; CMZ, ciliary marginal zone; Cce, cerebellum; GCL, ganglion cell layer; hb, hindbrain; H, hypothalamus; INL, inner nuclear layer; int, intestine; P, pallial proliferation zone; PO, preoptic area; tpz, tectal proliferation zones; Anterior towards the left. Scale bars in H′ for G′ and H′, in J for all of others: 100 µm.

Expression Data

Gene:	pcna
Fish:	WT ddb1^m863/m863
Anatomical Terms:	brain proliferative region ciliary marginal zone
Stage Range:	Prim-15 to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	ddb1^m863/m863
Observed In:	brain proliferative region cerebellum apoptotic process ciliary marginal zone apoptotic process optic tectum apoptotic process
Stage:	Protruding-mouth

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One