Fig. 5

Zimp7 restricts organizer gene expression. Dorsal and ventral gene expression in embryos at shield (A–N), 5 hpf (O–Q) and 3-somite stage (R–T). Embryos were hybridized to specific marker genes boz (A and B), gsc (C–E), flh (F–H), vox (I–K), vent (L–N), ntl (O–Q) and hgg1 (R–T). The injected reagents are indicated at the left. boz, lateral views, dorsal to the right; gsc, and flh dorsal views with the animal pole oriented toward the top; vox, vent and ntl, animal pole views with dorsal to the right; hgg1, anterior view, dorsal side to the top. Arrows in I–N indicate the angle of marginal expression of ventral genes. An asterisk indicates the ectopic expression of vent in the ectoderm. U and V, graphs showing average values obtained from a number of embryos (indicated at the bottom of each bar) as the ones shown in A–T. U, the relative differences in mean expression area in MO and mRNA injected embryos compared to control embryos (horizontal line normalized to one) for the dorsal genes and hgg1. V, the average negative expression angle in MO and mRNA injected embryos compared to control embryos for the ventral genes vox and vent. The number of embryos with given appearance for ntl (Q) is 81% (n=21). zimp7 morphants display a significant increase in all dorsal genes as well as in the organizer-induced gene hgg1 and a reduction in ventral gene expression. Opposite effects are observed when zimp7 is overexpressed. Notably, besides an increased marginal angle in ventral genes, there is augmented ectodermal expression. ^Np<0.05; ^**p<0.01; ^***p<0.001.