FIGURE

Fig. 2

ID

ZDB-FIG-150811-7

Publication

Moreno-Ayala et al., 2015 - PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development

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Fig. 2

Zimp7 loss-of-function causes axial phenotypes. (A) Embryos injected with a control MO at 9 hpf; relative to this embryos. (B) zimp7-sp MO injected embryos present an elongated shape. (C) After 30 hpf of development, zimp7-sp MO injected embryos are ventrally curved. (D) Co-injection of embryos with the zimp7-sp MO and the p53 MO rescued head defects but not axial defects. (E) At 30 hpf embryos injected with a zimp7-ATG MO present a similar phenotype. (F) Embryos with specific mutations in the zimp7 gene produced by the CRISPR/Cas system present a curly down tail and a similar appearance as the zimp7-sp MO injected embryos. (G) Distribution of phenotypes produced with the injected morpholinos. Total numbers of embryos are shown at the top.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + CRISPR1-zmiz2 WT + MO1-zmiz2 WT + MO1-zmiz2 + MO4-tp53 WT + MO2-zmiz2
Knockdown Reagents:	CRISPR1-zmiz2 MO1-zmiz2 MO2-zmiz2 MO4-tp53
Observed In:	head cell death notochord whole organism
Stage Range:	90%-epiboly to Prim-15

Phenotype Detail

Acknowledgments

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Reprinted from Developmental Biology, 403(1), Moreno-Ayala, R., Schnabel, D., Salas-Vidal, E., Lomelí, H., PIAS-like protein Zimp7 is required for the restriction of the Zebrafish organizer and mesoderm development, 89-100, Copyright (2015) with permission from Elsevier. Full text @ Dev. Biol.