Fig. 1

Antagonistic actions of Hox PG1 and Nlz factors on krox20 expression and initiation. All embryos were subjected to ISH with a krox20 probe. (A-D) Wild-type (WT) or Tg(hsp:hoxb1a) embryos were heat-shocked (HS) for 5, 20 or 30min at 100% epiboly and collected at 20s (three independent experiments). (E-H) WT embryos were injected with a control morpholino (moCtrl) or morpholinos for hoxb1a and hoxb1b (moHoxb1) and collected at 1s or 4s (three independent experiments). (I,J) krox20^fh227/fh227 mutant embryos were injected with 50ng/µl of control or hoxb1a mRNAs and collected at 3s. (K,L) krox20^fh227/fh227 mutant embryos were injected with a control morpholino (moCtrl) or morpholinos for hoxb1a and hoxb1b (moHoxb1) and collected at 3s. (M-P) WT or krox20^fh227/fh227 mutant embryos were injected with a control morpholino or morpholinos for nlz1 and nlz2 (moNlz) and collected at 3s. Embryos were flat-mounted with the anterior towards the top (see also Figs 2-6).