FIGURE

Fig. 5

ID

ZDB-FIG-150805-5

Publication

Labalette et al., 2015 - Molecular dissection of segment formation in the developing hindbrain

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Fig. 5

Regulation of enhancer C activity by Fgf signalling. (A-D) Tg(cC:gfp) embryos were treated with control DMSO carrier or SU5402 from the shield stage (A,B), or injected with a control morpholino (moCtrl) or a morpholino for spry4 (moSpry4), and analysed by ISH for gfp (three independent experiments). (E-H) Analysis of enhancer C activity in Tg(cC:gfp) and Tg(cCmutSp:gfp) embryos by single (E,F) or double (G,H) ISH for gfp (purple) and krox20 (orange) at 2s. (I-L) Tg(cCmutSp:gfp) embryos were treated with DMSO carrier or SU5402 from the shield stage (I,J), or injected with a control morpholino (K) or a morpholino for spry4 (S), and analysed by ISH for gfp (three independent experiments). In A,B,I,J, coloration reactions were performed with the Fast Red fluorescent compound to allow for quantification. ISHs were carried out in parallel on siblings under identical conditions.

Expression Data

Gene:	GFP
Fish:	zf1077Tg zf1078Tg zf1077Tg + MO3-spry4 zf1078Tg + MO3-spry4
Condition:	chemical treatment: SU5402
Knockdown Reagent:	MO3-spry4
Anatomical Terms:	presumptive rhombomere 3 presumptive rhombomere 4
Stage:	1-4 somites

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	zf1077Tg zf1078Tg zf1077Tg + MO3-spry4 zf1078Tg + MO3-spry4
Condition:	chemical treatment: SU5402
Knockdown Reagent:	MO3-spry4
Observed In:	presumptive rhombomere 3 presumptive rhombomere 4
Stage:	1-4 somites

Phenotype Detail

Acknowledgments

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