FIGURE

Fig. S1

ID

ZDB-FIG-150708-15

Publication

Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

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Fig. S1

gata2a is expressed within the vasculature, and is required for vascular integrity. (A) In situ hybridization of gata2a expression in the PLM at the 8-somite stage (13 hpf) (B) Expression of gata2a at 25 hpf. (C) Confocal microscopy shows a lateral view of the trunk region of gata2a:GFP;kdrl:mCherry^mem at 25 hpf. (C′) Digitally reconstructed transverse view of the trunk region in (C). (D-E) Pooling of red blood cells observed by o-dianisidine staining in 2 dpf siblings (D) and gata2a^um27 mutants (E). (F) Confocal microscopy of the head vasculature and erythrocytes in a 2 dpf lmo2:GFP;gata1a:DsRed;gata2a^um27-/- embryo. White arrows denote extravascular erythrocytes.

Expression Data

Genes:	DsRed EGFP gata2a mCherry
Fish:	WT la3Tg; s896Tg gata2a^um27/um27; sd2Tg; zf72Tg
Anatomical Terms:	cranial vasculature nucleate erythrocyte posterior lateral mesoderm vascular endothelium
Stage Range:	5-9 somites to Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	gata2a^um27/um27 gata2a^um27/um27; sd2Tg; zf72Tg
Observed In:	cranial vasculature head nucleate erythrocyte trunk
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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