FIGURE

Fig. S4

ID

ZDB-FIG-150708-18

Publication

Butko et al., 2015 - Gata2b is a restricted early regulator of hemogenic endothelium in the zebrafish embryo

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Fig. S4

gata2b morphants have normal primitive hematopoiesis, morphogenesis, and gata2a expression. (A-R) Whole mount in situ hybridization of wildtype embryos and gata2b morphant siblings. Expression of etsrp at 10 ss (A, B). (C-H) Expression of gata1a (C, D), scl (E, F), and runx1 (G, H) in 12 ss embryos. (I-N) Expression of gata1a (I,J), scl (K, L) and l-plastin (M, N) in 22 hpf embryos. (O, P) Expression of myod in 25 hpf embryos. (Q, R) Expression of cdh17 in 36 hpf embryos. (S, T) Expression of gata2a in 25 hpf control (S) and gata2b morphant embryos (T).

Expression Data

Genes:	cdh17 etsrp gata1a gata2b lcp1 myod1 runx1 tal1
Fish:	WT WT + MO1-gata2b
Knockdown Reagent:	MO1-gata2b
Anatomical Terms:	erythroid lineage cell macrophage posterior lateral mesoderm pronephric duct somite ventral wall of dorsal aorta
Stage Range:	10-13 somites to Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-gata2b
Knockdown Reagent:	MO1-gata2b
Observed In:	posterior lateral mesoderm primitive hemopoiesis pronephric duct development somite development
Stage Range:	10-13 somites to Prim-25

Phenotype Detail

Acknowledgments

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