Fig. 4

gata2b⁺ cells give rise to adult blood. (A,B) Analysis of GFP⁺ cells in the whole kidney marrow (WKM). (A) Representative flow cytometry analysis of the adult WKM, including the distribution of erythroid (red), lymphoid (blue), precursor (purple), myeloid (green) and eosinophil (orange) populations. (B) Percentage and distribution of GFP⁺ cells from the WKM of adult zebrafish of the indicated genotypes. Scatter plots depict the forward and side scatter distribution of GFP⁺ cells from the WKM, with populations marked as in A for reference. For wild type (n=1), gata2a:GFP (n=2) and UAS:GFP (n=2), the indicated percentage corresponds to the representative scatter plot. For gata2b:Gal4;UAS:GFP (n=3) and gata2b:Gal4;UAS:lifeactGFP (n=3), a representative scatter plot is shown and the indicated percentage of GFP⁺ cells corresponds to the average±s.d. (C-E′) gata2b:Gal4;UAS:Cre;βactin:Switch-DsRed embryo at 3dpf. (C,C′) DsRed fluorescence in the whole embryo. (D,D′) Enlarged view of thymus (T) and kidney (K) region from C,C&prime& P, pigment. (E,E′) Enlarged view of the CHT region from C,C′. (F) Representative flow cytometry analysis of adult WKM. (G) DsRed fluorescence of erythroid, lymphoid, precursor and myeloid fractions of WKM of βactin:Switch-DsRed (gray filled) and kdrl:Cre;βactin:Switch-DsRed (black line). (H) DsRed fluorescence of erythroid, lymphoid, precursor and myeloid fractions of WKM of UAS:Cre;βactin:Switch-DsRed (gray filled) and gata2b:Gal4;UAS:Cre;βactin:Switch-DsRed (black line). (I) Representative flow cytometry analysis of the adult spleen. (J) DsRed fluorescence of erythroid and lymphoid fractions of the spleen of βactin:Switch-DsRed (gray filled) and kdrl:Cre;βactin:Switch-DsRed (black line). (K) DsRed fluorescence of erythroid and lymphoid fractions of the spleen of UAS:Cre;βactin:Switch-DsRed (gray filled) and gata2b:Gal4;UAS:Cre;βactin:Switch-DsRed (black line). (G,H,J,K) Numbers indicate percentage of cells in the DsRed⁺ fraction.