ZFIN Image: Butko et al., 2015, Fig. S1

Image

Figure Caption

Fig. S1

gata2a is expressed within the vasculature, and is required for vascular integrity. (A) In situ hybridization of gata2a expression in the PLM at the 8-somite stage (13 hpf) (B) Expression of gata2a at 25 hpf. (C) Confocal microscopy shows a lateral view of the trunk region of gata2a:GFP;kdrl:mCherry^mem at 25 hpf. (C′) Digitally reconstructed transverse view of the trunk region in (C). (D-E) Pooling of red blood cells observed by o-dianisidine staining in 2 dpf siblings (D) and gata2a^um27 mutants (E). (F) Confocal microscopy of the head vasculature and erythrocytes in a 2 dpf lmo2:GFP;gata1a:DsRed;gata2a^um27-/- embryo. White arrows denote extravascular erythrocytes.

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Acknowledgments

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