FIGURE

Fig. 1

ID

ZDB-FIG-150427-41

Publication

Choe et al., 2015 - Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics

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Fig. 1

Eph-ephrin and pak2a expression in pouches. (A) Schematic of pouches and expression of efnb2a, efnb3b, ephb4a and pak2a within the 30hpf zebrafish head. (B-M) Fluorescent in situ hybridization (green) shows a time-course of expression for efnb2a, efnb3b, ephb4a and pak2a relative to her5:GFP⁺ endodermal pouches (labeled red by GFP immunohistochemistry). Arrows show expression in pouch endoderm and arrowheads show expression in neural-crest-derived cells. Scale bar: 40µm. (N-O′) Anti-Efnb2a staining (green) relative to her5:mCherryCAAX⁺ endoderm (red) shows increased expression in more mature anterior pouches (e.g. p3) relative to less mature posterior ones (p4 and p5), as well as in neural-crest-derived cells. Dotted lines in O′ denote pouch expression. Scale bar: 20µm.

Expression Data

Genes:	efnb2a EGFP
Fish:	ne1911Tg
Anatomical Terms:	migratory neural crest cell pharyngeal pouch
Stage:	14-19 somites

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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