FIGURE

Fig. 3

ID

ZDB-FIG-150427-43

Publication

Choe et al., 2015 - Eph-Pak2a signaling regulates branching of the pharyngeal endoderm by inhibiting late-stage epithelial dynamics

Other Figures

All Figure Page

Back to All Figure Page

Fig. 3

Pouch disintegration in the absence of EphB-Pak2a signaling. (A-P) Confocal projections from time-lapse recordings show development of her5:mCherryCAAX⁺ pouches p4-p6 (red). In embryos with reduced Efnb2a/b3b, endoderm-specific expression of KD-Ephb4a or mutant for pak2a, cells break off from the epithelium (arrows) as pouches migrate outwards. Movies start at 25hpf (T0). (Q-T) Confocal sections show pouches labeled by Alcama immunohistochemistry at 34hpf. Whereas wild-type pouch epithelial cells form bilayers with a straight line of apical membranes (arrows in Q), apical membranes are variably disorganized in embryos with reduced Efnb2a/b3b, endoderm-specific expression of KD-Ephb4a or mutant for pak2a. Note dissociated pouch epithelial cells (arrow) in nkx2.3:Gal4VP16; UAS:KD-Ephb4a embryos (S) and regions of monolayered (arrow) and multilayered (arrowhead) epithelia in pak2a mutant pouches (T). Scale bars: 20µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Development