FIGURE

Fig. 4

ID

ZDB-FIG-150420-8

Publication

Paolini et al., 2015 - Asymmetric inheritance of the apical domain and self-renewal of retinal ganglion cell progenitors depend on Anillin function

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Fig. 4

anillin hypomorphic conditions affect the balance between symmetric and asymmetric cell division of RGC progenitors. (A,B) Frames from supplementary material Movies 10-13 showing examples of symmetric and asymmetric division outcomes in the CtrlMO (A) and anlnMO (B) conditions. GFP intensity differences for A/P and A/A divisions were quantified (P<0.05 for CtrlMO clones; P=0.92 for anlnMO clones). (C) Division (asterisk) generating two Cxcr4b-eGFP-positive daughters (cyan) (10h 10′). Each time frame represents a z-projection. (C′) A single z-stack. (D,E) Symmetric divisions increase from 30% in the CtrlMO to 85% in the anlnMO (two-tailed Fisher′s exact test, *P=0.011). Apical surface of the retinal neuroepithelium is to the top. Scale bars: 5µm in A,B; 5.5µm in C,C′.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	AB + MO1-anln AB + MO2-anln
Knockdown Reagents:	MO1-anln MO2-anln
Observed In:	retinal ganglion cell asymmetric cell division
Stage Range:	Prim-5 to Long-pec

Phenotype Detail

Acknowledgments

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