FIGURE

Fig. 5

ID

ZDB-FIG-150309-43

Publication

Freisinger et al., 2014 - Live Imaging and Gene Expression Analysis in Zebrafish Identifies a Link between Neutrophils and Epithelial to Mesenchymal Transition

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Fig. 5

Cscr2 is required for EMT related gene expression in HRas^V12 expressing epithelial cells.

(A–B) Fluorescent Z stack projections of live 3.5 dpf transgenic mpx:GFP (green neutrophils) control MO injected (A) and cxcr2 morphant (B). (C–D) Fluorescent Z stack projections of live 3.5 dpf transgenic mpeg:Dendra (green macrophages), control MO injected (C) and cxcr2 morphant (D). (E) Quantification of A–B (as a ratio of neutrophils per transformed cell) reveals a significant decrease in neutrophil recruitment to HRas^V12 expressing cells in cxcr2 MO injected larvae compared to control. (F) Quantification of C–D (as a ratio of macrophages per transformed cell) shows that macrophage numbers at HRas^V12 expressing cells in cxcr2 MO injected larvae is similar to macrophage numbers at HRas^V12 expressing cells in control larvae. (G) Quantitative RT-PCR (one representative graph shown n = 4) indicates a statistically significant decrease in mmp9 and slug transcripts in transformed cells from cxcr2 MO injected larvae when compared to control MO injected larvae. *** = P<.001, ns = not significant. Scale bar = 20 microns.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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