ZFIN Image: Freisinger et al., 2014, Fig. 5

Image

Figure Caption

Fig. 5 Cscr2 is required for EMT related gene expression in HRas^V12 expressing epithelial cells.

(A–B) Fluorescent Z stack projections of live 3.5 dpf transgenic mpx:GFP (green neutrophils) control MO injected (A) and cxcr2 morphant (B). (C–D) Fluorescent Z stack projections of live 3.5 dpf transgenic mpeg:Dendra (green macrophages), control MO injected (C) and cxcr2 morphant (D). (E) Quantification of A–B (as a ratio of neutrophils per transformed cell) reveals a significant decrease in neutrophil recruitment to HRas^V12 expressing cells in cxcr2 MO injected larvae compared to control. (F) Quantification of C–D (as a ratio of macrophages per transformed cell) shows that macrophage numbers at HRas^V12 expressing cells in cxcr2 MO injected larvae is similar to macrophage numbers at HRas^V12 expressing cells in control larvae. (G) Quantitative RT-PCR (one representative graph shown n = 4) indicates a statistically significant decrease in mmp9 and slug transcripts in transformed cells from cxcr2 MO injected larvae when compared to control MO injected larvae. *** = P<.001, ns = not significant. Scale bar = 20 microns.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ PLoS One