FIGURE

Fig. 3

ID

ZDB-FIG-150309-41

Publication

Freisinger et al., 2014 - Live Imaging and Gene Expression Analysis in Zebrafish Identifies a Link between Neutrophils and Epithelial to Mesenchymal Transition

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Fig. 3

Leukocytes are recruited to HRas^V12 expressing epithelial cells.

(A–B) Analysis of time-lapse movies of control HRas^WT expressing epithelial cells (A and D) and HRas^V12 expressing epithelial cells (B and E) in 3.5 dpf transgenic mpx:GFP (green neutrophils) larvae (A–B) and 3.5 dpf transgenic mpeg:Dendra (green macrophage) larvae (D–E). For cell tracks leukocyte migration was tracked every 2 minutes for 30 minutes. (C) Quantification of A–B (as a ratio of neutrophils per transformed cell) confirms a statistically significant increase in neutrophil recruitment to HRas^V12 expressing cells compared to HRas^WT expressing cells. (F) Quantification of D–E (as a ratio of macrophages per transformed cell) confirms a statistically significant increase in macrophage recruitment to HRas^V12 expressing cells when compared to macrophages recruited to HRas^WT expressing cells. dpf = days post fertilization, scale bar = 20 microns, *** = p<.001 **** = p<.0001.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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