Fig. 3

Fig. 3 Leukocytes are recruited to HRas^V12 expressing epithelial cells.

(A–B) Analysis of time-lapse movies of control HRas^WT expressing epithelial cells (A and D) and HRas^V12 expressing epithelial cells (B and E) in 3.5 dpf transgenic mpx:GFP (green neutrophils) larvae (A–B) and 3.5 dpf transgenic mpeg:Dendra (green macrophage) larvae (D–E). For cell tracks leukocyte migration was tracked every 2 minutes for 30 minutes. (C) Quantification of A–B (as a ratio of neutrophils per transformed cell) confirms a statistically significant increase in neutrophil recruitment to HRas^V12 expressing cells compared to HRas^WT expressing cells. (F) Quantification of D–E (as a ratio of macrophages per transformed cell) confirms a statistically significant increase in macrophage recruitment to HRas^V12 expressing cells when compared to macrophages recruited to HRas^WT expressing cells. dpf = days post fertilization, scale bar = 20 microns, *** = p<.001 **** = p<.0001.