FIGURE

Fig. 2

ID

ZDB-FIG-141016-8

Publication

Subramanian et al., 2014 - Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions

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Fig. 2

Tsp4b is required for muscle attachment.Whole mount immunostaining of 36 hpf Tsp4b-deficient embryos using anti-MHC (A, D, G; green) and anti-Tsp4b (B, E, H; red) and merged (C, F, I). (A-C) Injection of 0.32 ng tsp4b-MO eliminates Tsp4b protein at 72 hpf but myofibers attach. (D-F) Electrical stimulation (30 V) of these larvae causes muscle detachment. (G-I) Co-injection of tsp4b RNA (80 pg/embryo) rescues muscle attachment and Tsp4b localization. (J) Histogram showing muscle detachment in 76% (N = 79) of stimulated Tsp4b-deficient embryos (Chi squared test p-value<0.001). (K) Co-injection of tsp4b mRNA rescues muscle attachment in 67% (N = 92) of stimulated Tsp4b-deficient embryos (Chi squared test p-value<0.001). (p-value representation legend: significant *<0.05, highly significant **<0.01, extremely significant ***<0.001, extremely significant ****<0.0001). Scale bar = 30 microns.

Expression Data

Gene:	thbs4b
Antibodies:	Ab-A4.1025 Ab1-thbs4b
Fish:	WT + MO1-thbs4b + MO1-tp53
Condition:	mechanical stress
Knockdown Reagents:	MO1-thbs4b MO1-tp53
Anatomical Term:	skeletal muscle cell
Stage:	Prim-25

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	WT + MO1-thbs4b + MO1-tp53
Condition:	mechanical stress
Knockdown Reagents:	MO1-thbs4b MO1-tp53
Observed In:	muscle somite somite border muscle tendon junction tendon
Stage:	Prim-25

Phenotype Detail

Acknowledgments

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