FIGURE

Fig. 1

ID

ZDB-FIG-141016-7

Publication

Subramanian et al., 2014 - Thrombospondin-4 controls matrix assembly during development and repair of myotendinous junctions

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Fig. 1

Zebrafish Tsp4b localizes to all muscle attachments.(A-L) Whole mount immunostaining of wild type embryos using anti-MHC (A, D, G, J; green) and anti-Tsp4b (B, E, H, K; red) and merged (C, F, I, L). (A-C) 20-22 hpf and (D-F) 72 hpf (lateral view) trunk showing early Tsp4b localization around notochord and medial somite boundaries (B and C) and later at somite boundaries (E and F). (G-L) Ventral (G-I) and lateral (J-L) views of 72 hpf showing Tsp4b at cranial muscle attachments. Abbreviations: AM-Adductor Mandibularis, AH-Adductor Hyoideus, AO-Adductor Operculae, DO-Dilator Operculae, HH-HyoHyal, IH-InterHyal, IMA-InterMandibularis Anterior, IMP-InterMandibularis Posterior, IO-Inferior Oblique, IR-Inferior Rectus, LAP-LevatorArcus Palatini, MR-Medial Rectus, SH-SternoHyoideus. Scale bar = 30 microns.

Expression Data

Gene:	thbs4b
Antibodies:	Ab-A4.1025 Ab1-thbs4b
Fish:	WT
Anatomical Terms:	adductor hyohyoid muscle tendon junction adductor mandibulae muscle tendon junction adductor operculi muscle tendon junction dilatator operculi muscle tendon junction head muscle heart hyohyoideus muscle tendon junction intermandibularis muscle tendon junction levator arcus palatini muscle tendon junction medial rectus muscle tendon junction notochord muscle tendon junction skeletal muscle cell somite border sternohyoid muscle tendon junction ventral intermandibularis anterior muscle tendon junction ventral intermandibularis posterior muscle tendon junction ventral oblique extraocular muscle muscle tendon junction ventral rectus muscle tendon junction
Stage Range:	20-25 somites to Protruding-mouth

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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