Fig. 7
High levels of neutrophil tyrosine nitration are attenuated by live wild type orΔRD1 Mm, but not heat killed Mm. (A) Example fluorescence confocal z-stacks of the caudal vein region of dominant active hif-1α (DA1) mRNA injected embryos stained with anti-nitrotyrosine antibody, imaged 1 day after injection with live, heat killed or ΔRD1 Mm. (B) Corrected fluorescence intensity levels of anti-nitrotyrosine antibody confocal z-stacks of equal size on dominant active hif-1α (DA1) mRNA injected embryos, 1 day after injection with live, heat killed or ΔRD1 Mm. Data shown are mean ± SEM, n = 74-108 cells from 15 embryos combined from 3 independent experiments. |