FIGURE

Fig. 3

ID

ZDB-FIG-140710-11

Publication

Walsh et al., 2011 - Planar polarity pathway and Nance-Horan syndrome-like 1b have essential cell-autonomous functions in neuronal migration

Other Figures

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Fig. 3

Nhsl1b interacts genetically and physically with Scrib in the regulation of facial branchiomotor (FBM) neuron migration. (A,B) Wild-type (A) and double heterozygous nhsl1b^fh131/+; scrib^rw468/+ zebrafish embryos (B) at 48 hours post-fertilization (hpf). Double heterozygotes have unmigrated FBM neurons in rhombomere (r)4, indicative of a strong genetic interaction between the two genes. (C) Histogram of phenotypes in the genotypic classes arising from a nhsl1b^fh131/+ x scrib^rw468/+ cross. (D-G) Nhsl1b associates with Scrib and Psd95 (Dlg4). cDNA constructs were transfected into HEK293T cells as indicated. Whole cell lysates were immunoprecipitated (IP) and western-blotted (WB) with the indicated antibodies. Scale bar: 20 µm.

Expression Data

Gene:	GFP
Fish:	rw0Tg (AB) nhsl1b^fh131/+; scrib^rw468/+; rw0Tg
Anatomical Terms:	branchiomotor neuron facial nerve motor nucleus rhombomere 4 facial nerve motor nucleus rhombomere 5 facial nerve motor nucleus rhombomere 6 facial nerve motor nucleus trigeminal motor nucleus
Stage:	Long-pec

Expression Detail

Antibody Labeling

Phenotype Data

Fish:	nhsl1b^fh131/+; scrib^rw468/+; rw0Tg
Observed In:	facial nerve motor nucleus facial nerve motor nucleus cell migration rhombomere 4
Stage:	Long-pec

Phenotype Detail

Acknowledgments

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