FIGURE

Fig. 2

ID

ZDB-FIG-140530-4

Publication

Blanco-Sánchez et al., 2014 - Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis

Other Figures

All Figure Page

Back to All Figure Page

Fig. 2

Cdh23 and Harmonin require Ift88 and Myo7aa for normal localization in hair cells. Immunolabeling in (A-D) wild-type siblings or (E-H) cdh23, (I-L) ush1c, (M-P) ift88 and (Q-T) myo7aa mutants of (A,E,I,M,Q) Cdh23, (B,F,J,N,R) Harmonin, (C,G,K,O,S) Ift88 and (D,H,L,P,T) Myo7aa. Confocal views of anterior macula hair cells are shown. Confocal sections are 0.8 µm thick, whereas the neck and basal region of the hair cell have an estimated diameter of 2-2.5 µm and 5-6 µm, respectively. Thus, not all confocal sections contain a hair bundle, and the observed shape of the hair bundle varies according to the mounting angle (see also supplementary material Fig. S3). Some individual hair cells are outlined by dotted lines. WT, wild type. Scale bar: 5 µm.

Expression Data

Genes:	ift88 ush1c
Antibodies:	Ab1-cdh23 Ab1-ift88 Ab2-myo7a
Fish:	WT cdh23^tj264/tj264 ift88^tz288/tz288 myo7aa^ty220/ty220 ush1c^fh293/fh293
Anatomical Term:	hair cell
Stage:	Day 5

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Dis. Model. Mech.