FIGURE

Fig. S8

ID

ZDB-FIG-140530-15

Publication

Blanco-Sánchez et al., 2014 - Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis

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Fig. S8

Proximity ligation control: Assay of nonspecific binding between proximity ligation probes in the absence of one of the primary antibodies during the reaction in WT conditions. (A,D,G,J,K,M,P) The proximity ligation reaction was performed with only one primary antibody: Goat anti-Cdh23 (A), rabbit anti-Harmonin (D), goat anti-Harmonin (G), rabbit anti-Ift88 (J), mouse anti-Myo7a (K), goat anti-Sec13 (M), or rabbit anti-Sec23 (P). (B,E,H,N,Q) single DIC channel showing a lateral view of the anterior macula. (C,F,I,L,O,R) Merged panels. Nonspecific signal was detected when only one primary antibody was added to the reaction. Scale bar: 5µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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