FIGURE

Fig. S5

ID

ZDB-FIG-140530-13

Publication

Blanco-Sánchez et al., 2014 - Complexes of Usher proteins preassemble at the endoplasmic reticulum and are required for trafficking and ER homeostasis

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Fig. S5

Cdh23 can reach the stereocilia in myo7aa mutants. (A,C) Specificity test for chicken anti-Cdh23 in wild-type sibling (A) and cdh23tj264a zebrafish mutant (C) using the AB complex detection method. A reminiscent weak signal is observed in the cdh23 mutant. This indicates that the tj264a mutation does not affect all cadherin 23 isoforms. (B,C) Cdh23 can be detected in the stereocilia of wild-type sibling (B) and myo7aa mutant (C) using a direct detection method.. In the direct detection conditions, quantification of the protein amount cannot be done due to saturation of the photodetector or the overall structure of the mechanoreceptor coupled to the lack of cytoplasmic signal in control experimental conditions. Arrowhead: hair bundle protein localization. Cell bodies are highlighted in different colors. Scale bar: 5µm.

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

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