FIGURE

Fig. 6

ID

ZDB-FIG-140428-21

Publication

Zhao et al., 2014 - Notch signaling regulates cardiomyocyte proliferation during zebrafish heart regeneration

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Fig. 6

Notch signaling is required for cardiomyocyte proliferation during zebrafish heart regeneration. (A and B) Representative cardiac sections from heat shocked control (CTRL) (A) and Tg(hsp70:DN-MAML) (B) animals carrying the Tg(gata4:DsRed2) transgene at 14 dpa. DsRed2 expression was visible in the compact myocardium of both control and Tg(hsp70:DN-MAML) hearts (n = 12/12 in each group). (C–F) Representative cardiac sections from heat shocked control (C and D) and Tg(hsp70:DN-MAML) (E and F) animals at 7 dpa. Sections were double immunostained to identify cardiomyocyte nuclei (Mef2+) and nuclei undergoing DNA replication (PCNA+). Boxed regions in C and E are shown at higher zoom in D and F. The percentages of myocardial nuclei undergoing DNA replication near the wound edges were quantified at 7 (G) and 14 (H) dpa and reported as mean proliferation indices ± 1 SD, **P < 0.01 and ***P < 0.001. In the control group, seven and eight hearts were analyzed at 7 and 14 dpa, respectively. In the Tg(hsp70:DN-MAML) group, three and four hearts were analyzed at 7 and 14 dpa, respectively.

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Antibody Labeling

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA