FIGURE

Fig. 5

ID

ZDB-FIG-140428-20

Publication

Zhao et al., 2014 - Notch signaling regulates cardiomyocyte proliferation during zebrafish heart regeneration

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Fig. 5

Regenerated coronary endothelium derives from preexisting endothelial cells during zebrafish heart regeneration. (A–D) Permanent labeling of endothelial cells during zebrafish embryogenesis. Tails of 96-hpf Tg(kdrl:CreER); Tg(ubi:Switch) embryos treated between 24 and 48 hpf with EtOH (A and B) or 4-HT (C and D) and imaged in the green (A and C) and red (B and D) channels. All 4-HT–treated embryos examined expressed mCherry in endothelial cells (n > 100), whereas EtOH-treated embryos failed to report red fluorescence (n > 100). (E–G) The adult endocardium and coronary endothelium displayed widespread and permanent mCherry labeling following 4-HT treatment during embryonic stages (n = 3/3). Representative cardiac section (E) and close up views of mCherry-labeled endocardium (F) and coronary endothelium (G). (H and I) Representative cardiac section from a regenerating Tg(kdrl:CreER); Tg(ubi:Switch) animal with mCherry-labeled endocardial and endothelial cells at 14 dpa. Numerous mCherry+ endothelial tubes (Inset) populated the regenerating myocardium. (n = 3/3.)

Expression Data

Expression Detail

Antibody Labeling

Phenotype Data

Phenotype Detail

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Proc. Natl. Acad. Sci. USA